Cre/loxP system Gene trap mice of the Gpr65 gene with a sleeping beauty transposable element. Homozygous mice are viable and fertile, but they show abnormal macrophage physiology and osteoclastic activity. 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Mol. Cell Biol., 23, 9189-9207 (2003). FASEB J., 28, 871-879 (2014). <br>研究成果の公表にあたって謝辞の表明を必要とする。 B6.Cg-Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>/Sati B6.Cg-Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>/Sati C (3-6 months) mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Phage P1 loxP sites, Salmon Sleeping Beauty transposase, Human bc1-2 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, Rabbit beta-globin adenylation signal sequence, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, Mouse Hprt splice donor (SD), mRNA instability (human granulocyte-macrophagecolony-stimulating factor cDNA) RBRC09690 大阪大学・竹田潤二教授により作製・分与（2003）、その後、石井聡先生がB6マウスに戻し交配。C57BL/6背景に9世代戻し交配済み。 Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 23, 9189-9207 (2003). FASEB J., 28, 871-879 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Fluorescent Proteins/lacZ System Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> Gpr65gt/gt Gpr65gt/gt true <a href='https://brc.riken.jp/mus/pcr09690'>Genotyping protocol -PCR-</a> Developed by Junji Takeda, Osaka University in 2003. Backcrossed to C57BL/6 for 9 generations by Satoshi Ishii, Akita University Graduate School of Medicine. C（3〜6か月） G-protein coupled receptor 65 (Gpr65), 別名T-cell death-associated gene 8 (TDAG8) はプロトンを感知するGタンパク質共役型受容体(GPCR)である。Sleeping beauty transposonがBDF1背景の生殖細胞に導入され、Gpr65のイントロン1に挿入されている。挿入部位はAC_000034.1 (Mus musculus strain mixed chromosome 12, alternate assembly Mm_Celera), region 99498712。ホモ型で交配、出産、成長は正常。破骨細胞・マクロファージ・ミクログリアの機能亢進。